Recommended products for immunohistochemistry CAT.Īnti-β-Actin Mouse Monoclonal Antibody (1C7)Īnti-beta Actin Rabbit Polyclonal AntibodyĪnti-GAPDH Mouse Monoclonal Antibody (2B5)Īnti-β-Tubulin Mouse Monoclonal Antibody (3G6)Īnti-PCNA Mouse Monoclonal Antibody (1D7)Īnti-PCNA Mouse Monoclonal Antibody (1D7), HRP ConjugatedĪnti-Histone H3 Mouse Monoclonal Antibody (2D10)Ībbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. At last, wish you all a smooth experiment. Step 6: Organize the data, Edit→Copy, you can Copy the data to ExcelĪbove, this is the share of immunohistochemical analysis using Image J. Then there is the analysis of the individual in the picture ↓↓↓ The percentage of positive area was 3.658%. We only need to select the second file here. This prompt is whether to display all the picture results of the three files. Step 5: Analysis results: Analyze→Analyze Particles, tick tick the corresponding column as shown in the pictureĬlick OK after the check is completed, and select No in the dialog box. In the displayed measurement parameter setting option box, select the required measurement parameter: Area (the Area of the selected Area) Mean (Mean gray value of the selected area of the box) IntDen (Integrated Density) (total fluorescence intensity in the selected area) Area Fraction. Step 4: Set measurement parameters: Analyze→Set Measurements Slide the two slider below to change the marked area. Step 3: Mark the stained area：Image→Adjust→Threshold Select relatively clear pictures for subsequent analysis, and choose file 2 here. Files 1, 2, and 3 correspond to red, green, and blue respectively.
Step 2: Transform the Image format: Image→Type→RGB StackĪt this time, a scroll bar appears at the bottom of the picture. Step 1: Import Image: Open Image J, File→Open: Open the Image to be measured
#MEASURE INTENSITY IMAGEJ HOW TO#
Now let's introduce how to analyze immunohistochemistry by Image J. If we can skillfully use Image J, it will undoubtedly be very helpful for us to analyze the experimental results. As a scientific researcher, we face all kinds of maddening experimental data every day.
#MEASURE INTENSITY IMAGEJ SOFTWARE#
Image J software is completely free and easy to download. The second is to subtract the background and measure the integrated density of each dot. The first is to treat each row as a horizontal 'lane' and use ImageJ's gel analysis function. Today we're going to talk about Image J’s use in immunohistochemistry. There are two built in methods for analyzing a dot blot in ImageJ.
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